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Safeguarding Your Experiment: Chemical Handling Chemical handling, the crafting of liquid mixtures or solutions, is a common practice. Yet, creating these blends demands more than just combining substances; it necessitates unwavering adherence to safety principles. A lapse in protocol could introduce hazards, from minor irritations to severe chemical exposures. This discourse will illuminate critical safety … Read more
Chemical reactions are the foundation of life, influencing everything from the food we consume to the air we breathe. Whether it’s a car engine burning fuel, a cake baking in the oven, or our bodies converting food into energy, chemical reactions are constantly at work. These reactions can be broadly classified into Exothermic and Endothermic … Read more
Chromatography plays a crucial role in scientific research, especially when it comes to separating and purifying biomolecules. One of the most effective techniques in this field is ion exchange chromatography (IEC). This method is widely used in biochemistry, biotechnology, and pharmaceutical industries to isolate proteins, peptides, nucleic acids, and other charged molecules with high precision. … Read more
Size Exclusion Chromatography (SEC), also known as gel filtration chromatography or molecular sieve chromatography, is a highly effective analytical technique used to separate molecules based on their size. It is widely used in biochemistry, biotechnology, and polymer chemistry to purify and analyze large biomolecules such as proteins, nucleic acids, and polymers. This article will cover … Read more
Gas chromatography (GC) is a powerful chemical technique widely used to separate, identify, and quantify volatile compounds in a mixture. Understand the intricate aroma of a freshly brewed cup of coffee. It’s not just “coffee it’s a symphony of volatile compounds, each contributing its unique note. How do we pull apart these invisible players and … Read more
Serial dilution is a widely used laboratory technique in microbiology, chemistry, and biology for reducing the concentration of a solution or suspension in a stepwise manner. This technique is essential for accurately analyzing and quantifying microorganisms, chemical substances, or other particles in a sample. This article will explore the principles, methods, and practical applications of … Read more
Column chromatography is a prevalent method utilized in the fields of chemistry and biochemistry for the separation and purification of distinct substances from a mixture. The technique is based on the principle of varying adsorption, where the components of the mixture interact differently with a stationary phase (usually a solid adsorbent) and a mobile phase … Read more
What are the principles of Thin-layer Chromatography [TLC]? Thin-layer chromatography (TLC) is an extensively employed analytical technique designed to separate and identify various components within a mixture. It bears a resemblance to paper chromatography; however, TLC utilizes a thin layer of adsorbent substances, like silica gel or alumina, applied to a plate made from glass, … Read more
Chromatography is a multifaceted technique employed to separate and examine mixtures and their components. Below are various types of chromatography, each possessing distinct applications and underlying principles: It works by distributing the components of a mixture between two phases: a stationary phase (which stays in place) and a mobile phase (which moves through or across … Read more
Separating Pigments: Lab Experiment Have you ever wondered how plants get their vibrant colors or what makes a flower petal so strikingly beautiful? The answer lies in pigments, natural compounds that absorb and reflect light. With a simple and engaging lab experiment called chalk chromatography, you can uncover the hidden colors within plant leaves, flowers, … Read more