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Ion Exchange Chromatography: A Powerful Technique for Biomolecule Separation Chromatography plays a crucial role in scientific research, especially when it comes to separating and purifying biomolecules. One of the most effective techniques in this field is ion exchange chromatography (IEC). This method is widely used in biochemistry, biotechnology, and pharmaceutical industries to isolate proteins, peptides, … Read more
Principles, Methods, and Practical Applications Size Exclusion Chromatography (SEC), also known as gel filtration chromatography or molecular sieve chromatography, is a highly effective analytical technique used to separate molecules based on their size. It is widely used in biochemistry, biotechnology, and polymer chemistry to purify and analyze large biomolecules such as proteins, nucleic acids, and … Read more
Principles, Methods, and Practical Applications Gas chromatography (GC) is a powerful chemical technique widely used to separate, identify, and quantify volatile compounds in a mixture. Understand the intricate aroma of a freshly brewed cup of coffee. It’s not just “coffee it’s a symphony of volatile compounds, each contributing its unique note. How do we pull … Read more
Principles, Methods, and Practical Applications Serial dilution is a widely used laboratory technique in microbiology, chemistry, and biology for reducing the concentration of a solution or suspension in a stepwise manner. This technique is essential for accurately analyzing and quantifying microorganisms, chemical substances, or other particles in a sample. This article will explore the principles, … Read more
High-Performance Liquid Chromatography Principles, Methods, and Practical Applications High-performance liquid Chromatography (HPLC) is an advanced analytical technique used to separate, identify, and quantify components in a mixture. It operates by pumping a liquid mobile phase at high pressure through a column packed with a stationary phase, separating compounds based on their interactions with the stationary … Read more
Principles, Types, and Applications. What is Column Chromatography? Column chromatography is a widely used separation technique in chemistry and biochemistry for isolating and purifying individual components from a mixture. It operates on the principle of differential adsorption, where compounds in a mixture interact differently with a stationary phase (typically a solid adsorbent) and a mobile … Read more
What are the principles and procedures of Thin-layer Chromatography [TLC]? Thin-layer chromatography (TLC) is a widely used analytical technique for separating and identifying components in a mixture. It is similar to paper chromatography but uses a thin layer of adsorbent material, such as silica gel or alumina, coated on a glass, plastic, or metal plate … Read more
Applications & Principles Chromatography is a versatile technique used to separate and analyze mixtures and components. Here are the different types of chromatography, each with its unique applications and principles It works by distributing the components of a mixture between two phases: a stationary phase (which stays in place) and a mobile phase (which moves through … Read more
Separating Pigments: Lab Experiment. Discovering the Colors of Nature Have you ever wondered how plants get their vibrant colors or what makes a flower petal so strikingly beautiful? The answer lies in pigments, natural compounds that absorb and reflect light. With a simple and engaging lab experiment called chalk chromatography, you can uncover the hidden … Read more
Introduction Paper chromatography is a simple yet powerful technique used for the separation of mixture substances. Whether paper chromatography is used in a chemistry lab, forensic investigations, or the food industry, this method plays a crucial role in analyzing components in a mixture. In this article, we’ll explore the principles of paper chromatography, its uses, … Read more