Size Exclusion Chromatography (SEC), also known as gel filtration chromatography or molecular sieve chromatography, is a highly effective analytical technique used to separate molecules based on their size. It is widely used in biochemistry, biotechnology, and polymer chemistry to purify and analyze large biomolecules such as proteins, nucleic acids, and polymers. This article will cover … Read more

Gas chromatography (GC) is a powerful analytical technique widely used in chemistry to separate, identify, and quantify volatile compounds in a mixture. It plays a critical role in various industries, including pharmaceuticals, environmental analysis, food and beverage, and forensic science. This article will discuss the principles, methods, and practical applications of gas chromatography in chemistry. … Read more

Serial dilution is a widely used laboratory technique in microbiology, chemistry, and biology for reducing the concentration of a solution or suspension in a stepwise manner. This technique is essential for accurately analyzing and quantifying microorganisms, chemical substances, or other particles in a sample. This article will explore the principles, methods, and practical applications of … Read more

High-performance liquid Chromatography (HPLC) is an advanced analytical technique used to separate, identify, and quantify components in a mixture. It operates by pumping a liquid mobile phase at high pressure through a column packed with a stationary phase, separating compounds based on their interactions with the stationary phase. HPLC is widely used in pharmaceuticals, environmental … Read more

What is Column Chromatography? Column chromatography is a widely used separation technique in chemistry and biochemistry for isolating and purifying individual components from a mixture. It operates on the principle of differential adsorption, where compounds in a mixture interact differently with a stationary phase (typically a solid adsorbent) and a mobile phase (a liquid or … Read more

What are the principles and procedures of Thin-layer Chromatography [TLC]? Thin-layer chromatography (TLC) is a widely used analytical technique for separating and identifying components in a mixture. It is similar to paper chromatography but uses a thin layer of adsorbent material, such as silica gel or alumina, coated on a glass, plastic, or metal plate … Read more

Discovering the Colors of Nature Have you ever wondered how plants get their vibrant colors or what makes a flower petal so strikingly beautiful? The answer lies in pigments, natural compounds that absorb and reflect light. With a simple and engaging lab experiment called chalk chromatography, you can uncover the hidden colors within plant leaves, … Read more